In Vitro Conservation of Plant Species
Indiscriminate harvest, poor perpetuity in nature, and lack of efficiency with conventional propagation methods, pose major limitations in the conservation and restoration of many plant species. In vitro cell and tissue culture can facilitate a continuous supply of germplasm for re-planting, selection of elite individuals, development of disease resistance, eradication of systemic infections, long term conservation of genetic resources and sustainable use of plant biodiversity. Development of technologies for multiplication of plants in controlled environments is a key element in GRIPP’s approach to conservation, genetic improvement, and potential commercialization of plant species.
GRIPP currently maintains an in vitro germplasm collection comprised of over 60 species from around the globe, including a variety of endangered medicinal plants and native Canadian plants. Some of the species currently being investigated include native trees such as American elm, American chestnut, maple, oak, ash and cherry birch.
- Murch, S.J., D. Ragone, W.L. Shi, A.R. Alan, P.K. Saxena (2008). In Vitro conservation and sustained production of breadfruit (Artocarpus altilis, Moraceae): Modern technologies to improve distribution of a traditional tropical crop. Naturwissenschaften, 95(2):99-107.
- He, Shan-shan, Chun-zhao Liu, and Praveen K. Saxena (2007). "Plant regeneration of an endangered medicinal plant Hydrastis canadensis L." Scientia horticulturae 113 (1) : 82-86.
- Liu, Chun-Zhao, et al. (2004) "Goldenseal (Hydrastis canadensis L.): in vitro regeneration for germplasm conservation and elimination of heavy metal contamination." In Vitro Cellular & Developmental Biology-Plant 40 (1) : 75-79.
- Murch, SJ, et al. (2004). "In vitro conservation and propagation of medicinal plants." Biodiversity 5 (2): 19-24.