Micropropagation combined with large-scale multiplication in bioreactors, and cultivation in growth chambers and greenhouses can be used to achieve plant growth with stable and optimum development and chemical profile. This integrated approach has important applications in the rapid production and distribution of plants for the purposes of conservation by replantation and reforestation. Based on past work done at the PCTL, GRIPP has developed efficient mass multiplication protocols to conserve and multiply plants that are chemically consistent and free of biological and environmental pollutants. A number of these protocols involve the use of temporary liquid immersion bioreactor systems which are very conducive for large-scale multiplication of plant species. One of GRIPP’s goals is to design and optimize new semi-automated temporary liquid immersion bioreactor systems that increase in vitro plant propagation efficiency, biomass accumulation, and meet the requirements of large scale multiplication of plant species meant for replanting. Additionally, controlled environment production offers a unique opportunity for fast introduction of novel hybrids, mutants, sports, native species, and plants with improved traits such as unique flower color, disease resistance, altered yield, and higher content of medicinally active compounds.
- Abbasi, B.H., R. Liu, P.K. Saxena, C.Z. Liu. (2009). Cichoric acid production from hairy root cultures of Echinacea purpurea grown in a modified airlift bioreactor. J. Chem. Tech. & Biotechnology 84: 1697-1701.