The Gosling Institute for Plant Preservation Logo Footer
University of Guelph


Bovey Bldg
601 Gordon St, Guelph, ON
N1G 1Y2 Canada

Production Systems

GRIPP Plant Production Systems

Micropropagation is a process commonly employed in the horticultural industry, whereby a single plant is grown using plant tissue culture and then multiplied hundreds, if not thousands of times to produce genetically identical clones of itself. This process combined with advanced plant tissue culture technologies such as bioreactors, has enabled large scale cultivation of plants which are generally hard to propagate through conventional approaches (i.e. sowing seed, vegetative cuttings).

Our integrated approach which combines micropropagation and environmentally controlled greenhouse technology has important applications in the rapid production and distribution of threatened and endangered plants by allowing researchers to restore wild populations at risk of extinction at a much quicker rate.

Additionally, controlled environment production offers a unique opportunity for fast introduction of commercially important plants such as novel hybrids, mutants, sports, native species, and plants with improved traits such as unique flower color, disease resistance, altered yield, and higher content of medicinally active compounds.

Selected Publications

  1. Shukla MR, Amritpal SS, Piunno K, Saxena PK, Jones AMP. (2017). Application of 3D printing to prototype and develop novel plant tissue culture systems. Plant Methods. 13(1): 6.
  2. Amritpal SS, Jones AMP, Shukla MR, Saxena PK. (2017). High light intensity stress as the limiting factor in micropropagation of sugar maple (Acer saccharum Marsh.). Plant Cell, Tissue and Organ Culture 129(2): 209-221.
  3. Salama A, Shukla MR, Popova E, Fisk N, Jones AMP, Saxena PK. (2017). In vitro propagation and re-introduction of Golden Paintbrush (Castilleja levisecta), a critically imperiled plant species. Canadian Journal of Plant Science.
  4. Padmanabhan P, Murch SJ, Saxena PK. (2015). Micropropagation of Primulina dryas (Dunn) Mich. Möller & A. Webber: High frequency regeneration from leaf explants. In Vitro Cellular & Developmental Biology 192(2015): 250-255.
  5. Latawa J, Shukla MR, Saxena PK. (2015). An efficient temporary immersion system for micropropagation of hybrid hazelnut. Botany. 94(1): 1-8.
  6. Zhao Y, Sun W, Wang Y, Saxena PK, Liu CZ. (2012). Improved mass multiplication of Rhodiola crenulata shoots using temporary immersion bioreactors with forced ventilation. Applied Biochemistry and Biotechnology. 166: 1480-1490.